1.11 Summary

    1. 1 light microscopy showshow cells move and behave
    2. 2 electron microscopy showshow complexes arrange in cells
    3. 3 single particle reconstruction showshow proteins arrange in complexes
    4. 4 X-ray crystallography showshow atoms arrange in proteins
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    Summary

    Concept Check Questions

    • How can you locate a protein of interest by light microscopy?

    • What happens to liquid water in a vacuum?

    • What are gold beads used for in cryo-ET?

    • Which technique can reveal the atomic structure of a small protein?

    Further Reading

    • Jensen. Getting Started in Cryo-EM Video Lectures [15].

    • Oikonomou and Jensen (2017). Cellular electron cryotomography: toward structural biology in situ [16].

    • Ruska (1987). Nobel Lecture: The development of the electron microscope and of electron microscopy [17].

    • Tsien (2005). Breeding molecules to spy on cells [18].